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Erin Green

Assoc Prof

Biological Sciences
Biological Sciences, Room 325
Stanford University (2013)
Ph D, University of California, Berkeley (2007)
BA, Bryn Mawr College (2000)
Research Interests

Research in our lab aims to understand how post-translational modifications of proteins direct epigenetic and cellular signaling pathways to regulate key biological functions, including the establishment of proper states of gene expression and the ability of cells to respond to stress. Histones, the primary protein component of chromatin, are subject to many types of post-translational modification, including acetylation, phosphorylation and methylation. These modifications are critical to controlling the accessibility of DNA during essential processes such as transcription and DNA repair.

We are specifically interested in methylation of histone lysine residues, a modification system that has been well-established to regulate chromatin structure and function. Aberrant regulation of histone lysine methylation leads to the disruption of chromatin homeostasis and has been implicated in numerous human pathologies, including tumorigenesis. There remain many unanswered questions regarding the functional and mechanistic details of both canonical and novel sites of histone methylation. Additionally, the existence of non-histone protein methylation is emerging as a key regulator of nuclear signaling pathways, but the extent and function of these methylation events are largely unknown.

Our primary research objectives are to (1) identify new mechanisms of chromatin regulation mediated by novel histone methylation events and (2) develop a comprehensive understanding of lysine methylation as a broad regulator of nuclear signaling pathways. We use budding yeast as a model system, integrating molecular biology, genetics, biochemistry, genomics and proteomics. The evolutionary conservation of many of the players involved in lysine methylation signaling allows our work to be broadly applicable to higher eukaryotes, and will provide insight in to the role of these factors in diverse human diseases.

Selected Classes
Fall 2016 BIOL 303 – Cell Biology
Fall 2015 BIOL 426 – Appr To Molecular Biol
Contracts, Fellowships, Grants, and Sponsored Research

Green, Erin M. The SMYD lysine methyltransferase Set6 in proteostasis and signaling Grant (Funded) Sponsored by National Institutes of Health (Aug 1, 2019 – Mar 31, 2021)

Green, Erin M. Lysine methylation at chromatin and cellular responses to stress Grant (Funded) Sponsored by National Institutes of Health (Jul 1, 2017 – Jun 30, 2022)

Green, Erin M. Mechanisms of chromatin homeostasis at telomeres mediated by histone lysine methyltransferases Grant (Funded) Sponsored by National Institutes of Health (NIA) (Jan 1, 2016 – Dec 31, 2016)

Intellectual Contributions

Function of the MYND Domain and C-Terminal Region in Regulating the Subcellular Localization and Catalytic Activity of the SMYD Family Lysine Methyltransferase Set5. 2 vol. 40 Molecular and cellular biology

Using yeast to define the regulatory role of protein methylation. Current protein & peptide science

Histone Modifications and the Maintenance of Telomere Integrity. 2 vol. 8 Cells

Assessing Yeast Cell Survival Following Hydrogen Peroxide Exposure. 2 vol. 9 Bio-protocol

SET domains and stress: uncovering new functions for yeast Set4. Current genetics

Set4 is a chromatin-associated protein, promotes survival during oxidative stress, and regulates stress response genes in yeast. 37 vol. 293 14429-14443 The Journal of biological chemistry

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae. 130 Journal of visualized experiments : JoVE

Repression of Middle Sporulation Genes in Saccharomyces cerevisiae by the Sum1-Rfm1-Hst1 Complex Is Maintained by Set1 and H3K4 Methylation. 12 vol. 7 3971-3982 G3 (Bethesda, Md.)

Choose Your Own Adventure: The Role of Histone Modifications in Yeast Cell Fate. vol. 429 1946-1957 Journal of molecular biology

The histone methyltransferases Set5 and Set1 have overlapping functions in gene silencing and telomere maintenance. 2 vol. 12 93-104 Epigenetics

Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons. 4 vol. 9 Epigenetics

Proteome-wide enrichment of proteins modified by lysine methylation. 1 vol. 9 37-50 Nature protocols

New marks on the block: Set5 methylates H4 lysines 5, 8 and 12. 4 vol. 3 335-9 Nucleus (Austin, Tex.)

A negative feedback loop at the nuclear periphery regulates GAL gene expression. 7 vol. 23 1367-75 Molecular biology of the cell

Methylation of H4 lysines 5, 8 and 12 by yeast Set5 calibrates chromatin stress responses. 3 vol. 19 361-3 Nature structural & molecular biology

Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae. 1 vol. 187 9-19 Genetics

Members of the H3K4 trimethylation complex regulate lifespan in a germline-dependent manner in C. elegans. 7304 vol. 466 383-7 Nature

Replication-independent histone deposition by the HIR complex and Asf1. 22 vol. 15 2044-9 Current biology : CB